SV40 Protocols by Leda Raptis (English) Hardcover Book

Description: SV40 Protocols by Leda Raptis Later work showed that SV40 DNA sequences as well as infectious virus are in fact found in human tumors and may have contributed to oncog- esis. FORMAT Hardcover LANGUAGE English CONDITION Brand New Publisher Description The initial incentive for in-depth study of Simian Virus 40 biology originated from the revelation of public health implications its use had in the 1960s. SV40 gained notoriety at this time when it was found to be a contaminant of polio and adenovirus vaccines that had been distributed to millions of healthy individuals worldwide. SV40 Protocols presents a consolidation of numerous well-tested step-by-step techniques from experts with hands-on experience. The book is organized in two sections. The first relates to assays of the lytic cycle of the virus, starting with a valuable introductory chapter on basic techniques for growth of SV40 and its mutants in tissue culture. Techniques for the growth and detection of two SV40-related human viruses, BKV and JCV, are also presented for those involved in clinical research. The second section begins with detailed biological assays of neoplastic transformation that are applicable to any system. All of the detailed and thorough chapters include discussions of problems and pitfalls that may be encountered, as well as troubleshooting advice.This comprehensive volume of optimzied experimental protocols will assist the beginner, as well as more experience researchers, to over come problems of ambiguity often present in SV40 literature. Back Cover Extensive research has shown that Simian Virus 40, a contaminant of polio and adenovirus vaccines that may be implicated in human cancers, can also serve as a powerful probe for examining many fundamental questions in molecular biology. In SV40 Protocols, Leda Raptis and a panel of highly experienced investigators describe in step-by-step fashion key techniques for experimentally detecting SV40 in human tumors, for exploiting its use in human gene therapy, and for studying its replication and its mechanisms of neoplastic transformation. Included are methods for growing SV40 and its related viruses in tissue culture, for in vivo and in vitro replication and transcription of SV40 DNA, for the use of retroviral vectors to express SV40 tumor antigens in cultured cells, and for transgenic mouse models based on the SV40 large T antigen. All methods have been optimized for experimental success, and the authors provide cogent discussions of the problems and pitfalls that may be encountered, as well as valuable troubleshooting advice. An appendix lists all companies whose products are cited in the text and includes an Internet directory for locating other reagent sources. Detailed and highly practical, SV40 Protocols offers both clinical and basic researchers powerful, well-tested tools for research on SV40 replication and neoplastic transformation, as well as techniques for its detection in human tumors and for creating and using powerful new gene therapy vectors. Table of Contents Propagation of Wild-Type and Mutant SV40.- Propagation and Assay of the JC Virus.- Propagation and Assay of BK Virus.- Molecular Methods for Identification and Genotyping of BK Virus.- Methods for Studying Interactions Between Simian Virus 40 T-Antigen and the Viral Origin of Replication.- Cell-Free Transcription of SV40.- Quantitation and Structural Analysis of SV40 RNAs.- Generation of Recombinant SV40 Vectors for Gene Transfer.- Production of SV40 Proteins in Insect Cells and In Vitro Packaging of Virions and Pseudovirions.- Using Retroviral Vectors to Express SV40 Tumor Antigens.- Neoplastic Transformation Assays.- Methods to Assess Inhibition of Adipocyte Differentiation by SV40 Large T Antigen.- SV40-Mediated Immortalization.- Biology of p53 and SV40 Large T Association.- Binding of SV40 Large T Antigen to the Retinoblastoma Susceptibility Gene Product and Related Proteins.- In situ Electroporation for the Measurement of c-Ras Activation by SVLT.- Role of SV40 Small t in Cell Lysis, Transformation, and Signaling.- Cytotoxic T Lymphocytes in SV40 Infections.- Detection of SV40 DNA Sequences in Human Tissue.- Guide to Techniques in Creating Transgenic Mouse Models Using SV40 T Antigen. Long Description Simian virus 40 gained notoriety in the 1960s because it was found to be a contaminant of polio and adenovirus vaccines that had been administered to millions of healthy individuals worldwide. The public health implications of this revelation provided the initial impetus for an in-depth study of SV40 biology. Later work showed that SV40 DNA sequences as well as infectious virus are in fact found in human tumors and may have contributed to oncog- esis. It also turned out that SV40 uses mostly cellular machinery to carry out many steps in viral infection, which makes it a powerful probe for examining many fundamental questions in eukaryotic molecular biology. SV40 Pro- cols consolidates a number of well-tested step-by-step techniques in one v- ume; experts with hands-on experience in particular methods give detailed accounts of their optimized experimental protocols, so that the beginner, as well as more experienced researchers, may readily overcome problems of ambiguity often present in the literature. As with other DNA tumor viruses, the response of cultured cells to SV40 infection depends upon the species being infected. Monkey cells s- port virus production, which leads to their death, whereas rodent cells p- duce only the early proteins and acquire a transformed phenotype. Thus, SV40 Protocols is organized in two sections. The first relates to assays of the lytic cycle of the virus, and the second deals with transformation. Feature Includes supplementary material: sn.pub/extras Details ISBN0896036537 Language English ISBN-10 0896036537 ISBN-13 9780896036536 Media Book Format Hardcover Series Number 165 Place of Publication Totowa, NJ Country of Publication United States Edited by Leda Raptis Author Leda Raptis Imprint Humana Press Inc. Short Title SV40 PROTOCOLS 2001/E Pages 310 DOI 10.1226/0896036537;10.1007/978-1-59259-117-6;10.1385/1592591175 AU Release Date 2000-12-26 NZ Release Date 2000-12-26 US Release Date 2000-12-26 UK Release Date 2000-12-26 Publisher Humana Press Inc. Edition Description 2001 ed. Series Methods in Molecular Biology Year 2000 Edition 2001st Publication Date 2000-12-26 Alternative 9781489942074 DEWEY 572.8 Illustrations XI, 310 p. Audience Undergraduate We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! 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