Pharmacogenomics: Methods and Protocols by Federico Innocenti (English) Paperbac

Description: Pharmacogenomics by Federico Innocenti Highlights include techniques for making a snapshot of the allele-specific variation in human gene expression, genome wide analysis of allele-specific expression using oligo microarrays, in vivo assays with HaploChIP, SNP genotyping in DNA pools, and PharmGKB, the pharmacogenetics and pharmacogenomics knowledge base. FORMAT Paperback LANGUAGE English CONDITION Brand New Publisher Description Expert researchers and physician/clinicians describe in detail the newest and most commonly used technologies today in this rapidly advancing field. The authors provide readily reproducible methods for assessing the functional consequences of a certain polymorphism, evaluate the variety of genotyping platforms currently available, and discuss the management of pharmacogenomic information. Highlights include techniques for making a snapshot of the allele-specific variation in human gene expression, genome wide analysis of allele-specific expression using oligo microarrays, in vivo assays with HaploChIP, SNP genotyping in DNA pools, and PharmGKB, the pharmacogenetics and pharmacogenomics knowledge base. The methodologies for genotyping include denaturing high-performance liquid chromarography, pyrosequencing, kinetic-fluorescence detection, mass spectrometry, and TaqMan assay for insertion/deletions. Back Cover Pharmacogenomics holds the promise of drugs tailor-made to an individuals genetic makeup for greater efficacy and safety. In Pharmacogenomics: Methods and Protocols, expert researchers and physician/clinicians describe in detail the newest and most common technologies now used in this rapidly advancing field. The authors provide readily reproducible methods for assessing the functional consequences of a certain polymorphism, evaluate the variety of genotyping platforms currently available, and discuss the management of pharmacogenomic information. Highlights include techniques for making a snapshot of the allele-specific variation in human gene expression, genome wide analysis of allele-specific expression using oligo microarrays, in vivo assays with HaploChIP, SNP genotyping in DNA pools, and PharmGKB, the pharmacogenetics and pharmacogenomics knowledge base. The methodologies for genotyping include denaturing high-performance liquid chromatography, pyrosequencing, kinetic-fluorescence detection, mass spectrometry, and TaqMan assay for insertion/deletions. The protocols follow the successful Methods in Molecular Biology(tm) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Pharmacogenomics: Methods and Protocols offers pharmacologists, geneticists, molecular biologists, and clinical chemists working in academia, biotechnology, and the pharmaceutical industry a cutting-edge collection of the key techniques used for todays preclinical and clinical studies. Table of Contents History and Overview.- Pharmacogenomics.- Functional Analysis of Gene Variation.- Transfection Assays With Allele-Specific Constructs.- Snapshot of the Allele-Specific Variation in Human Gene Expression.- Genome-Wide Analysis of Allele-Specific Gene Expression Using Oligo Microarrays.- HaploChIP.- Genotyping Techniques.- Aspects Influencing Genotyping Method Selection.- Denaturing High-Performance Liquid Chromatography for Mutation Detection and Genotyping.- Pyrosequencing of Clinically Relevant Polymorphisms.- Kinetic Fluorescence-Quenching Detection Assay for Allele Frequency Estimation.- Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.- Fluorescence-Based Fragment Size Analysis.- Single-Nucleotide Polymorphism Genotyping in DNA Pools.- TaqMan Genotyping of Insertion/Deletion Polymorphisms.- Management of Pharmacogenomic Information.- PharmGKB.- Systems for the Management of Pharmacogenomic Information. Long Description Expert researchers and physician/clinicians describe in detail the newest and most commonly used technologies today in this rapidly advancing field. The authors provide readily reproducible methods for assessing the functional consequences of a certain polymorphism, evaluate the variety of genotyping platforms currently available, and discuss the management of pharmacogenomic information. Highlights include techniques for making a snapshot of the allele-specific variation in human gene expression, genome wide analysis of allele-specific expression using oligo microarrays, in vivo assays with HaploChIP, SNP genotyping in DNA pools, and PharmGKB, the pharmacogenetics and pharmacogenomics knowledge base. The methodologies for genotyping include denaturing high-performance liquid chromarography, pyrosequencing, kinetic-fluorescence detection, mass spectrometry, and TaqMan assay for insertion/deletions. Details ISBN1617375713 Publisher Humana Press Inc. Series Methods in Molecular Biology Year 2010 Edition 1st ISBN-10 1617375713 ISBN-13 9781617375712 Format Paperback Imprint Humana Press Inc. Place of Publication Totowa, NJ Country of Publication United States Edited by Federico Innocenti DEWEY 615.7 Short Title PHARMACOGENOMICS Language English Media Book Series Number 311 Subtitle Methods and Protocols Pages 214 Illustrations X, 214 p. DOI 10.1007/978-1-59259-957-8 Publication Date 2010-11-19 AU Release Date 2010-11-19 NZ Release Date 2010-11-19 US Release Date 2010-11-19 UK Release Date 2010-11-19 Author Federico Innocenti Edition Description Softcover reprint of hardcover 1st ed. 2005 Alternative 9781588294401 Audience Professional & Vocational We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! TheNile_Item_ID:96232451;

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