Description: Metabolomics by Wolfram Weckwerth Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic analysis. Metabolomics: Methods and Protocols contains forward-looking protocols, which provide the essential groundwork for future efforts in elucidating the structure of the unknowns detected in metabolomic studies. FORMAT Paperback LANGUAGE English CONDITION Brand New Publisher Description Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic analysis. Leading researchers in the field present protocols for the application of complementary analytical methods, such as gas chromatography-mass spectrometry (GC-MS). Metabolomics: Methods and Protocols contains forward-looking protocols, which provide the essential groundwork for future efforts in elucidating the structure of the unknowns detected in metabolomic studies. Back Cover Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic analysis. Leading researchers in the field present protocols for the application of complementary analytical methods, such as gas chromatography-mass spectrometry (GC-MS). Metabolomics: Methods and Protocols contains forward-looking protocols, which provide the essential groundwork for future efforts in elucidating the structure of the unknowns detected in metabolomic studies. Part I of this volume focuses on GC-MS techniques; specifically, attention is given to metabolite profiling in blood plasma, construction and application of mass spectral and retention time index libraries, and metabolomic profiling of natural volatiles. In Part II, emphasis shifts to data integration and data mining techniques, and specific protocols discuss improving pattern recognition and biomarker selection for systems level approaches, and visualization and analysis of molecular and metabolomic data. Parts III, IV, and V examine the coupling of capillary electrophoresis and mass spectrometry, classical reversed phase liquid chromatography-mass spectrometry, and electrochemical detection of metabolites using HPLC separations coupled with coulometric electrode array detectors. In Part VI, protocols for the determination of metabolic flux rates are presented and a theoretical framework for metabolic pathway network analysis is provided. The volume concludes with an examination of cutting-edge NMR protocols. Table of Contents Metabolome Analysis Using Gas Chromatography Coupled to Mass Spectrometry.- Metabolite Profiling in Blood Plasma.- Nonsupervised Construction and Application of Mass Spectral and Retention Time Index Libraries From Time-of-Flight Gas Chromatography-Mass Spectrometry Metabolite Profiles.- Metabolomic Profiling of Natural Volatiles.- Metabolomics: Data Integration and Data Mining.- Integrative Profiling of Metabolites and Proteins.- Integrating Profiling Data.- Visualization and Analysis of Molecular Data.- A Gentle Guide to the Analysis of Metabolomic Data.- Capillary Electrophoresis Coupled to Mass Spectrometry.- Capillary Electrophoresis-Mass Spectrometry for Metabolomics.- Liquid Chromatography Coupled to Mass Spectrometry for Metabolomics and Structural Elucidation.- Application of Liquid Chromatography-Mass Spectrometry Analysis in Metabolomics.- Electrochemical Detection of Metabolites.- High-Performance Liquid Chromatography Separations Coupled With Coulometric Electrode Array Detectors.- Metabolic Fluxes.- Determination of Metabolic Flux Ratios From 13C-Experiments and Gas Chromatography-Mass Spectrometry Data.- Understanding the Roadmap of Metabolism by Pathway Analysis.- NMR Metabolome Analysis.- Revealing the Metabolome of Animal Tissues Using 1H Nuclear Magnetic Resonance Spectroscopy.- Nuclear Magnetic Resonance Metabonomics.- Practical Aspects of Uniform Stable Isotope Labeling of Higher Plants for Heteronuclear NMR-Based Metabolomics. Review From the reviews:"This book forms part of an extensive and successful series from Methods in Molecular Biology … . this book provides a good introduction to metabolomics, with useful protocols and relevant data analysis techniques that can be adapted easily to suit any researchers needs." (Elizabeth Want, ChemMedChem, Issue 2, 2007) Long Description Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic analysis. Leading researchers in the field present protocols for the application of complementary analytical methods, such as gas chromatography-mass spectrometry (GC-MS). Metabolomics: Methods and Protocols contains forward-looking protocols, which provide the essential groundwork for future efforts in elucidating the structure of the unknowns detected in metabolomic studies. Part I of this volume focuses on GC-MS techniques; specifically, attention is given to metabolite profiling in blood plasma, construction and application of mass spectral and retention time index libraries, and metabolomic profiling of natural volatiles. In Part II, emphasis shifts to data integration and data mining techniques, and specific protocols discuss improving pattern recognition and biomarker selection for systems level approaches, and visualization and analysis of molecular and metabolomic data. Parts III, IV, and V examine the coupling of capillary electrophoresis and mass spectrometry, classical reversed phase liquid chromatography-mass spectrometry, and electrochemical detection of metabolites using HPLC separations coupled with coulometric electrode array detectors. In Part VI, protocols for the determination of metabolic flux rates are presented and a theoretical framework for metabolic pathway network analysis is provided. The volume concludes with an examination of cutting-edge NMR protocols. Review Quote From the reviews:"This book forms part of an extensive and successful series from Methods in Molecular Biology … . this book provides a good introduction to metabolomics, with useful protocols and relevant data analysis techniques that can be adapted easily to suit any researchers needs." (Elizabeth Want, ChemMedChem, Issue 2, 2007) Feature Includes supplementary material: sn.pub/extras Details ISBN1617376434 Publisher Humana Press Inc. Year 2010 ISBN-10 1617376434 ISBN-13 9781617376436 Format Paperback Edition 1st Imprint Humana Press Inc. Place of Publication Totowa, NJ Country of Publication United States Edited by Wolfram Weckwerth DEWEY 612.39078 Short Title METABOLOMICS Language English Media Book Series Number 358 Subtitle Methods and Protocols Author Wolfram Weckwerth Illustrations 8 Illustrations, color; 71 Illustrations, black and white; 312 p. 79 illus., 8 illus. in color. Pages 312 AU Release Date 2010-11-19 NZ Release Date 2010-11-19 US Release Date 2010-11-19 UK Release Date 2010-11-19 Edition Description Softcover reprint of hardcover 1st ed. 2007 Series Methods in Molecular Biology Publication Date 2010-11-19 Alternative 9781588295613 Audience Professional & Vocational We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! TheNile_Item_ID:96238118;
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