Legionella: Methods and Protocols (Methods in Molecular Biology)

Description: This Methods in Molecular Biology(TM) series book presents methods specifically adapted and developed for the study of distinct features of L. pneumophila. Includes materials lists, reproducible protocols, and notes on troubleshooting and pitfalls. Part 1. Identification and Typing of Legionella in the Environment and During Disease 1. Identification of Legionella in the Environment Natalia A. Kozak, Claressa E. Lucas, and Jonas M. Winchell 2. Identification of Legionella in Clinical Samples Sophe Jarraud, Ghislaine Descours, Christophe Ginevra, Gerard Lina, and Jerome Etienne 3. Sequence-Based Identification of Legionella Rodney M. Ratcliff 4. Investigation of Outbreaks - Epidemiology Carol Joseph 5. Outbreak Investigations and Identification of Legionella in Contaminated Water Susanne Lee and John Lee 6. Typing Methods for Legionella Christian Lück, Norman K. Fry, Jürgen H. Helbig, Sophie Jarraud, and Timothy G. Harrison Part 2. Physiology and genetics of Legionella 7. Culture, Media and Handling of Legionella Christa H. Chatfield and Nicholas P. Cianciotto 8. The Intracellular Metabolism of Legionella by Isotopologue Profiling Klaus Heuner and Wolfgang Eisenreich 9. Induction of Competence for Natural Transformation in Legionella pneumophila and Exploitation for Mutant Construction Carmen Buchrieser and Xavier Charpentier 10. Constructing Unmarked Gene Deletions in Legionella pneumophila Andrew Bryan, Zachary D. Abbot, and Michele Swanson 11. Natural Biofilm Formation with Legionella pneumophila Emilie Portier and Yann Héchard 12. Production of Legionella pneumophila Monospecies Biofilms in a Low-Nutrient Concentration Medium Sophie Pécastaings and Christine Roques 13. Enrichment of Outer Membrane Vesicles Shed by Legionella pneumophila Jens Jäger and Michael Steinert Part 3. Cellular microbiology of Legionella 14. Analysis of Legionella Infection by Flow Cytometry Andre Tiaden, Aline Kessler, and Hubert Hilbi 15. Analysis of Legionella Infection Using RNAi in Drosophila Cells Dennise A. De Jesús, Tamara J. O'Connor, and Ralph R. Isberg 16. Immunofluorescence Imaging of Legionella Infection In vitro Stefan S. Weber and Annette Oxenius 17. Electron Microscopy of Legionella and Legionella-Infected Cells Gary Faulkner and Raphael Garduno 18. Pathogen Vacuole Purification from Legionella-Infected Amoeba and Macrophages Christine Hoffmann, Ivo Finsel, and Hubert Hilbi 19. Methods for Determining Protein Translocation by the Legionella pneumophila Dot/Icm Type IV Secretion System Wenhan Zhu and Zhao-Qing Luo 20. Subcellular Localization of Legionella Dot/Icm Effectors Adam J. Vogrin, Aurelie Mousnier, Gad Frankel, and Elizabeth L. Hartland Part 4. Biochemical assays to study Legionella effectors and enzymes 21. Purification and Characterization of Legionella U-Box-Type E3 ubiquitin ligase Hiroki Nagai and Tomoko Kubori 22. Legionella Phospholipases Implicated in Infection - Determination of Enzymatic Activities Kathleen Seipel and Antje Flieger 23. Interactions of Legionella Effector Proteins with Host Phosphoinositide Lipids Stephen Weber, Stephanie Dolinsky, and Hubert Hilbi 24. Characterization of Legionella lipopolysaccharide Christian Lück and Jürgen Helbig Part 5. Immunity and host response against Legionella 25. The Tetrahymena and Acanthamoeba Model Systems Sharon G. Berk and Rafael A. Garduño 26. Dictyostelium Host Response to Legionella infection: Strategies and Assays Salvatore Bozzaro, Barbara Peracino, and Ludwig Eichinger 27. The Caenorhabditis elegans Model of Legionella Infection Ann Karen C. Brassinga and Costi D. Sifri 28. Infection of Nonphagocytic Host Cells by Legionella Shandra R. Day, Costi D. Sifri, and Paul S. Hoffman 29. Analyzing Caspase-1 Activation During Legionella pneumophila Infection in Macrophages Christopher L. Case and Craig R. Roy 30. The Mouse as a Model for Pulmonary Legionella Infection Juliana I. Hori and Dario Zamboni 31. Assessment of Legionella-Specific Immunity in Mice Stefan Weber, Nicole Joller, and Annette Oxenius 32. The Guinea Pig Model of Legionnaires' Disease Paul H. Edelstein 33. Human Susceptibility to Legionnaires¿ Disease William R. Berrington and Thomas R. Hawn Part 6. Genomics, transcriptomics and proteomics of Legionella 34. cDNA library Construction for Next Generation Sequencing (NGS) to Determine the Transcriptional Landscape of Legionella pneumophila Tobias Sahr and Carmen Buchrieser 35. Methods to Study Legionella Transcriptome in vitro and in vivo Sebastian Faucher and Howard Shuman 36. Co-Immunoprecipitation: Protein-RNA and Protein-DNA Interaction Tobias Sahr and Carmen Buchrieser 37. Identification of Legionella Effectors Using Bioinformatic Approaches Gil Segal Part 5. Immunity and host response against Legionella 25. The Tetrahymena and Acanthamoeba Model Systems Sharon G. Berk and Rafael A. Garduño 26. Dictyostelium Host Response to Legionella infection: Strategies and Assays Salvatore Bozzaro, Barbara Peracino, and Ludwig Eichinger 27. The Caenorhabditis elegans Model of Legionella Infection Ann Karen C. Brassinga and Costi D. Sifri 28. Infection of Nonphagocytic Host Cells by Legionella Shandra R. Day, Costi D. Sifri, and Paul S. Hoffman 29. Analyzing Caspase-1 Activation During Legionella pneumophila Infection in Macrophages Christopher L. Case and Craig R. Roy 30. The Mouse as a Model for Pulmonary Legionella Infection Juliana I. Hori and Dario Zamboni 31. Assessment of Legionella-Specific Immunity in Mice Stefan Weber, Nicole Joller, and Annette Oxenius 32. The Guinea Pig Model of Legionnaires' Disease Paul H. Edelstein 33. Human Susceptibility to Legionnaires¿ Disease William R. Berrington and Thomas R. Hawn Part 6. Genomics, transcriptomics and proteomics of Legionella 34. cDNA library Construction for Next Generation Sequencing (NGS) to Determine the Transcriptional Landscape of Legionella pneumophila Tobias Sahr and Carmen Buchrieser 35. Methods to Study Legionella Transcriptome in vitro and in vivo Sebastian Faucher and Howard Shuman 36. Co-Immunoprecipitation: Protein-RNA and Protein-DNA Interaction Tobias Sahr and Carmen Buchrieser 37. Identification of Legionella Effectors Using Bioinformatic Approaches Gil Segal

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