Legionella: Methods and Protocols by Carmen Buchrieser (English) Hardcover Book

Description: Legionella by Carmen Buchrieser, Hubert Hilbi The methods described in the second edition on Legionella are for the study of distinct features of L. pneumophila. Chapters guide readers through ecology and physiology of legionella, legionella genetics, cellular microbiology of legionella, biochemical assays to study legionella effectors and enzymes, immunity and host response against legionella, metagenomics, proteomics, and host microbiomes of legionella. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Legionella: Methods and Protocols, Second Edition aims to be useful for scientists studying Legionella, and for a broader research community interested in the ecology, physiology, pathogenesis, immunity, genetics and evolution of other bacterial pathogens. FORMAT Hardcover LANGUAGE English CONDITION Brand New Back Cover The methods described in the second edition on Legionella are for the study of distinct features of L. pneumophila . Chapters guide readers through ecology and physiology of legionella, legionella genetics, cellular microbiology of legionella, biochemical assays to study legionella effectors and enzymes, immunity and host response against legionella, metagenomics, proteomics, and host microbiomes of legionella. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Legionella: Methods and Protocols, Second Edition aims to be useful for scientists studying Legionella , and for a broader research community interested in the ecology, physiology, pathogenesis, immunity, genetics and evolution of other bacterial pathogens. Table of Contents Analysis of Iron Requirements and Siderophore Production.- The Pathometabolism of Legionella Studied by Isotopologue Profiling.- Quantification of Viable but Non-culturable Cells of Legionella pneumophila.- Selection of Legionella Virulence-related Traits by Environmental Protozoa.- Migration of Acanthamoeba castellanii through Legionella Biofilms.- Scar-free Genome Editing in Legionella pneumophila.- Querying Legionella Genomes using Transposition-sequencing.- Screening Targeted Legionella pneumophila Mutant Libraries in vivo using INSeq.- Genetic Manipulation of non-pneumophila Legionella: Protocols Developed for Legionella longbeachae.- Quantitative Imaging Flow Cytometry of Legionella-containing Vacuoles in Dually Fluorescence-labelled Dictyostelium.- Sorting of Phagocytic Cells Infected with Legionella pneumophila.- Single Cell Analysis of Legionella and Legionella-infected Acanthamoeba by Agarose Embedment.- Mitochondrial Dynamics and Activity in Legionella-Infected Cells.- Perturbation of Legionella Cell Infection by RNA Interference.- Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila.- In situ Imaging and Structure Determination of Bacterial Toxin Delivery Systems using Electron Cryotomography.- Methods for Non-canonical Ubiquitination and Deubiquitination Catalyzed by Legionella pneumophila Effector Proteins.- Purification and Analysis of Effector Glucosyltransferase Lgt1 from Legionella pneumophila.- Determination of in vivo Interactomes of Dot/Icm type IV Secretion System Effectors by Tandem Affinity Purification.- Inflammasome Activation in Legionella-infected Macrophages.- Infection of Human Lung Tissue Explants (HLTEs) with Legionella pneumophila.- The Galleria mellonella Infection Model for Investigating the Molecular Mechanisms of Legionella Virulence.- Dictyostelium Host Response to Legionella Infection: Strategies and Assays.- 6.1.3 The Caenorhabditis elegans model of Legionella infection.- The Mouse as a Model for Pulmonary Legionella Infection.- Targeted Metagenomics of Microbial Diversity in Free-living Amoebae and Water Samples.- Analyses of the Pulmonary Microbiome Composition of Legionella Pneumophila-Infected Patients.- Subcellular Protein Fractionation in Legionella pneumophila and Preparation of the Derived Sub-proteomes for Analysis by Mass Spectrometry. Feature Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Details ISBN1493990470 Publisher Humana Press Inc. Series Methods in Molecular Biology Year 2019 Edition 2nd ISBN-10 1493990470 ISBN-13 9781493990474 Format Hardcover Imprint Humana Press Inc. Subtitle Methods and Protocols Place of Publication Totowa, NJ Country of Publication United States Edited by Hubert Hilbi DEWEY 616.241 Pages 476 Publication Date 2019-01-29 Short Title Legionella Language English DOI 10.1007/978-1-4939-9048-1 Series Number 1921 AU Release Date 2019-01-29 NZ Release Date 2019-01-29 US Release Date 2019-01-29 UK Release Date 2019-01-29 Author Hubert Hilbi Translator Jurgita Antoine Birth 1974 Affiliation Univ Degli Studi Di Verona, Italy Position Customer Qualifications MD Edition Description 2nd ed. 2019 Audience Professional & Vocational Illustrations 50 Illustrations, color; 24 Illustrations, black and white; XXVI, 476 p. 74 illus., 50 illus. in color. We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! TheNile_Item_ID:130978116;

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