Extracellular Vesicles in Diagnosis and Therapy (Methods in Molecular Biology)

Description: This detailed book provides an exhaustive picture of current methods to detect, isolate, and analyze extracellular vesicles (EVs) from diverse sources, now seen as potential disease biomarkers as well as tools for the development of new therapies. Beginning with a section on detection and isolation of EVs, the volume continues with chapters covering different methods to isolate and quantify EVs from specialized tissues/organs and body fluids, methods devoted to analyzing EV components, as well as cutting-edge methods to engineer EVs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Extracellular Vesicles in Diagnosis and Therapy serves as an ideal guide for researchers seeking to learn more about the complexities of cell-to-cell communication. Part I: Extracellular Vesicles Detection and Isolation 1. Aptasensors for Cancerous Exosome Detection Jin Li, Sitao Xie, Fengli Qu, and Weihong Tan 2. Detection of Cancer-Derived Exosomes Using a Sensitive Colorimetric Aptasensor Lizhou Xu and Khuloud T. Al-Jamal 3. Isolation of Circulating Extracellular Vesicles by High-Performance Size-Exclusion Chromatography Kaloyan Takov, I-Jou Teng, and Manuel Mayr 4. Isolation and Proteomic Analysis of Mouse Serum Small Extracellular Vesicles for Individual Subject Analysis Federica Anastasi, Marialaura Dilillo, Davide Pellegrini, and Liam A. McDonnell 5. Protocol for Measuring Concentrations of Extracellular Vesicles in Human Blood Plasma with Flow Cytometry Najat Hajji, Chi M. Hau, Rienk Nieuwland, and Edwin van der Pol Part II: Isolation and Characterization of Tissue and Biofluid-Specific EVs 6. Targeted Mass Spectrometry-Based Proteomics Method to Quantify Placental Extracellular Vesicles Andrew Lai, Carlos Palma, Alexis Salas, Flavio Carrion, and Carlos Salomon 7. Isolation and Characterization of Extracellular Vesicles Secreted from Human Pluripotent Stem Cell-Derived Cardiovascular Progenitor Cells Qiang Wu, Min-Xia Ke, and Huang-Tian Yang 8. Isolation and Characterization of Salivary Exosomes for Cancer Biomarker Discovery Lucas Trevisan França de Lima, Juliana Müller Bark, Mohammad Rasheduzzaman, Chameera Ekanayake Weeramange, and Chamindie Punyadeera 9. Isolation and Characterization of Urinary Extracellular Vesicles for MicroRNA Biomarker Signature Development with Reference to MISEV Compliance Marlene Reithmair, Anja Lindemann, Veronika Mussack, and Michael W. Pfaffl Part III: Analysis of EV Components 10. Direct Detection of Extracellular Vesicle miRNAs Using a Single-Step RT-qPCR Assay Ayyanar Sivanantham, Heedoo Lee, and Yang Jin 11. Purification and Phosphoproteomic Analysis of Plasma-Derived Extracellular Vesicles Anton B. Iliuk 12. Lipidomic Analysis of Extracellular Vesicles Isolated from Human Plasma and Serum Yuchen Sun, Kosuke Saito, and Yoshiro Saito Part IV: EV Engineering 13. Extracellular Vesicles and their Use as Vehicles of Immunogens Chiara Chiozzini, Barbara Ridolfi, and Maurizio Federico 14. Isolation and Fluorescent Labeling of Extracellular Vesicles from Cultured Tumor Cells Noelle Leary, Sarina Walser, and Lothar C. Dieterich 15. Generation, Characterization, and Count of Fluorescent Extracellular Vesicles Flavia Ferrantelli, Valentina Tirelli, Valeria Barreca, and Francesco Manfredi 16. Optimized Protocol for Plasma-Derived Extracellular Vesicles Loading with Synthetic miRNA Mimic Using Electroporation Margherita A.C. Pomatto, Federica Negro, and Giovanni Camussi 17. Extracellular Vesicle Loading via pH-Gradient Modification Stephanie M. Kronstadt, Steven M. Jay, and Anjana Jeyaram

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Location: Gloucester

End Time: 2025-01-11T17:12:35.000Z

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