Description: Antiviral RNAi by Ronald P. van Rij Here is a collection of protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as for development and optimization of RNAi-based antiviral drugs. The methods here can also be applied outside the context of a virus infection. FORMAT Paperback LANGUAGE English CONDITION Brand New Publisher Description Viruses and RNAi share an intricate relationship at many levels. RNAi is an important antiviral defense mechanism in plants and invertebrates, microRNAs – of viral or cellular origin – affect many aspects of virus biology, and replication of many, if not all, mammalian viruses can be suppressed by RNAi. Antiviral RNAi: Concepts, Methods, and Applications provides a collection of protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as for the development and optimization of RNAi-based antiviral drugs. As RNAi is a central regulatory mechanism in the cell, the methods in this volume can also be applied out of the context of a virus infection. Divided into five convenient parts, this detailed volume reviews important basic concepts in the field of antiviral RNAi, provides experimental and bio-informatic tools for the analysis of small silencing RNAs, covers methods to biochemically dissect RNAi-based antiviral defense and viral counter-defense mechanisms, describes methods for the design, expression, and delivery of therapeutic antiviral siRNAs, and finally presents genome-wide RNAi approaches for the identification of factors involved in virus replication. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and accessible, Antiviral RNAi: Concepts, Methods, and Applications serves as an ideal guide for both novice and experienced researchers alike striving to dissect the role of RNAi in the viral life cycle or to further boost the development of novel therapeutics and experimental tools based on RNAi technology. Back Cover Viruses and RNAi share an intricate relationship at many levels. RNAi is an important antiviral defense mechanism in plants and invertebrates, microRNAs - of viral or cellular origin - affect many aspects of virus biology, and replication of many, if not all, mammalian viruses can be suppressed by RNAi. Antiviral RNAi: Concepts, Methods, and Applications provides a collection of protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as for the development and optimization of RNAi-based antiviral drugs. As RNAi is a central regulatory mechanism in the cell, the methods in this volume can also be applied out of the context of a virus infection. Divided into five convenient parts, this detailed volume reviews important basic concepts in the field of antiviral RNAi, provides experimental and bio-informatic tools for the analysis of small silencing RNAs, covers methods to biochemically dissect RNAi-based antiviral defense and viral counter-defense mechanisms, describes methods for the design, expression, and delivery of therapeutic antiviral siRNAs, and finally presents genome-wide RNAi approaches for the identification of factors involved in virus replication. Written in the highly successful Methods in Molecular Biology(tm) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Antiviral RNAi: Concepts, Methods, and Applications serves as an ideal guide for both novice and experienced researchers alike striving to dissect the role of RNAi in the viral life cycle or to further boost the development of novel therapeutics and experimental tools based on RNAi technology. Table of Contents Defense and counter-defense in the RNAi-based antiviral immune system in insects.- RNAi and cellular miRNAs in infections by mammalian viruses.- Viral miRNAs.- Progress in RNAi-based antiviral therapeutics.- Chemical modification of small interfering RNA.- Viral small RNA cloning and sequencing.- Visitor, an informatic pipeline for analysis of viral siRNA sequencing datasets.- Computational prediction of viral miRNAs.- Detection of Viral microRNAs by Northern Blot Analysis.- Detection of viral microRNA with S1 Nuclease Protection assay.- Characterization of RISC associated adenoviral small RNAs.- Identification of Viral Suppressors of RNAi by a Reporter Assay in Drosophila S2 Cell Culture.- Dicer assay in Drosophila S2 cell extract.- Slicer activity in Drosophila melanogaster S2 extract.- Gel Mobility Shift Assays for RNA-binding viral RNAi suppressors.- dsRNA uptake in adult Drosophila.- Design of small interfering RNAs for antiviral applications.- RNAi inducing lentiviral vectors for anti-HIV-1 gene therapy.- Production of multicopy shRNA lentiviral vectors for antiviral therapy.- Intranasal delivery of antiviral siRNA.- Antibody mediated delivery of siRNAs for anti-HIV therapy.- Aptamer-targeted RNAi for HIV-1 therapy.- RNAi screening for host factors involved in viral infection using Drosophila cells.- Genome-wide RNAi Screen for ViralReplication in Mammalian Cell Culture.- RNAi screening in mammalian cells to identify novel host cell molecules involved in the regulation of viral infections. Review From the reviews:"This well-illustrated book details the necessary protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as the development and optimization of RNAi-based antiviral drugs. The book is intended for both novice and experienced researchers … . It also will be of immense help to graduate students, physicians who treat patients with infectious diseases, and other researchers interested in the field of antiviral RNAi. … a well-illustrated and authoritative resource on important basic concepts in the field of antiviral RNAi." (Omer Iqbal, Doodys Review Service, July, 2011) Review Quote From the reviews: "This well-illustrated book details the necessary protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as the development and optimization of RNAi-based antiviral drugs. The book is intended for both novice and experienced researchers ... . It also will be of immense help to graduate students, physicians who treat patients with infectious diseases, and other researchers interested in the field of antiviral RNAi. ... a well-illustrated and authoritative resource on important basic concepts in the field of antiviral RNAi." (Omer Iqbal, Doodys Review Service, July, 2011) Feature Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Details ISBN1493958259 Pages 425 Publisher Humana Press Inc. Series Methods in Molecular Biology Year 2016 ISBN-10 1493958259 ISBN-13 9781493958252 Format Paperback Publication Date 2016-08-23 Imprint Humana Press Inc. Subtitle Concepts, Methods, and Applications Place of Publication Totowa, NJ Country of Publication United States Edited by Ronald P. van Rij DEWEY 616 Short Title Antiviral RNAi Language English Series Number 721 UK Release Date 2016-08-23 AU Release Date 2016-08-23 NZ Release Date 2016-08-23 US Release Date 2016-08-23 Author Ronald P. van Rij Edition Description Softcover reprint of the original 1st ed. 2011 Alternative 9781617790362 Audience Professional & Vocational Illustrations 18 Illustrations, color; 21 Illustrations, black and white; XI, 425 p. 39 illus., 18 illus. in color. We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! TheNile_Item_ID:126572699;
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ISBN-13: 9781493958252
Book Title: Antiviral RNAi
Item Height: 254 mm
Item Width: 178 mm
Author: Ronald P. Van Rij
Publication Name: Antiviral Rnai: concepts, Methods, and Applications
Format: Paperback
Language: English
Publisher: Humana Press Inc.
Subject: Medicine, Biology
Publication Year: 2016
Type: Textbook
Item Weight: 1097 g
Number of Pages: 425 Pages
