Description: A Laboratory Guide for in Vivo Studies of DNA Methylation and Protein/DNA Interactions (Biomethods) [Hardcover] Jost, J P and Saluz, H P Product Overview A Safety Considerations Genomie sequencing involves a number oj hazard- ous steps, such as high eurrent, high voltage, radioaetive and highly toxie chemieals. It is, the- jore, absolutely essential that the instruetions oj equipment manu/aeturers bejollowed and that par- tieular attention is paid to the loeal and jederal safety regulations. I Introduction 13 B Introduction Hypomethylation ofDNA has been positively correlated with thc activation of many eucaryotic genes. During the transition from inactive to active genes changes in the protein/DNA interaction pattern occur. Tran- scriptional activation of eucaryotic genes is mediated by specific interac- tions oftransacting factors with their respective DNA binding sites in Lhe control regions (promoters, enhancers) ofthe genes. This process is ofLen accompanied by changes in local chromatin strucLure, witnessed by the appearance of nuclease hypersensitive sites, as weil as by changes in protein-DNA interactions and, in the case of higher eucaryotes, alterations ofthe cytosine methylation pattern. The sole available experimental tech- nique that permits the study ofthe latter phenomena at single nucleotide resolution is direct genomic sequencing/footprinting, pioneered by Church and Gilbert (1984). This method combines the chemical DNA- sequencing procedure of Maxam amI Gilbert (1980) with thc detection 01' DNA sequences by electroblotting and indirect end-Iabeling by hybridiza- tl0n. An alternative possibility is the novel procedure (Saluz and . lost, 1989), using Taq polymerase. The first steps 01' both meLhods are essen- tially the total genomic DNA is digested wiLh a suilable restriction enzyme and the resulting DNA fragments are chemically sequeneed. Read more Details Publisher : Birkhauser (April 1, 1999) Language : English Hardcover : 286 pages ISBN-10 : 0817623698 ISBN-13 : 92 Item Weight : 1.58 pounds Dimensions : 7 x 0.75 x 9.75 inches Best Sellers Rank: #12,454,860 in Books (See Top 100 in Books) #4,449 in Cell Biology (Books) #4,449 in Cell Biology (Books) We have been selling used books since 2012, and we've learned that the most important thing is doing good business. Honesty is our policy. Free Shipping We ship worldwide. We have multiple warehouses around the world, so please note the extended handling time on certain listings.
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ISBN: 0817623698
ISBN10: 0817623698
ISBN13: 9780817623692
EAN: 9780817623692
MPN: does not apply
Brand: Birkhauser
GTIN: 09780817623692
Number of Pages: 286 Pages
Language: English
Publication Name: Laboratory Guide for in Vitro Studies of D N A Methylation and Protein-D N A Interactions
Publisher: Springer
Publication Year: 1990
Subject: Life Sciences / Molecular Biology, Life Sciences / Genetics & Genomics, Chemistry / Organic
Type: Textbook
Subject Area: Science
Author: Hans P. Saluz, Jurgen P. Jost
Series: Biomethods Ser.
Format: Hardcover